First evidence for the implication of inflammatory process in Ciguatera Fish Poisoning

First evidence for the implication of inflammatory process in Ciguatera Fish Poisoning
Matsui M, Kumar-Roine S, Darius HT, Chinain M, Laurent D, Pauillac S
Type de communication: 
Communication orale
Congrès Ciguatéra et Biotoxines associées (Nouméa, Nouvelle-Calédonie)

Ciguatera Fish Poisoning (CFP) is an ichtyosarcotoxism associated with marine ciguatoxins (CTXs) and represents a complex human pathology with gastro-intestinal, neurological, and cardio-vascular disorders.
This study provides the first experimental evidence that CTXs enhance the expression of pro- and anti-inflammatory cytokines and can also lead to overproduction of nitric oxide (NO) via the modulation of inducible NO synthase (iNOS).
The effects of P-CTX-1B versus bacterial lipopolysaccharide (LPS) on IL-1-β, IL-6, IL-10, IL-11, TNF-α and iNOS expression were investigated using RAW 264.7 cells. The respective mRNA levels were quantified using quantitative PCR while NO production was indirectly measured in the culture supernatants with Griess reagent.
P-CTX-1B led to overexpression of interleukin-1-β (IL-1-β), tumornecrosisfactor- α, IL-6 and IL-10. These cytokines expression profiles differed from that observed with LPS. In contrast, IL-11 was not influenced by P-CTX-1B, as opposed to LPS.
The iNOS mRNA expression was found to be up-regulated by incubation with P-CTX-1B. The resulting NO overproduction was evidenced by measurements of nitrites in the 10 µM range.
These new findings provide a better insight into the physiopathology of CFP and a likely explanation of the long-term persistence of certain symptoms, notably pruritus and Chronic Fatigue Syndrome. They also pave the way for novel research on traditional medicines used in CFP treatment.
Workshop international Ciguatéra et biotoxines associées (Nouméa, 27-31 octobre 2008)